1P35

CRYSTAL STRUCTURE OF BACULOVIRUS P35

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	114.580, 130.110, 181.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.196
Rwork	0.196
R-free	0.26000 *
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	2.040 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (0.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Rmerge	0.064
Total number of observations	324073 *
Number of reflections	66997 *
<I/σ(I)>	14.1
Completeness [%]	97.8
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	4 *	or batch *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir		1.5-2.0 (M)
3	1	reservoir		100 (mM)
4	1	reservoir	ethylene glycol	1 (%)