1P2R

T4 LYSOZYME CORE REPACKING MUTANT I78V/TA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-09
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	59.996, 59.996, 95.229
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.000 - 1.580
R-factor	0.191
Rwork	0.188
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.011
RMSD bond angle	2.300 *
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.600	1.670
High resolution limit [Å]	1.580 *	1.560
Rmerge	0.046	0.274
Number of reflections	26083
<I/σ(I)>	7.9	2.7
Completeness [%]	94.6	883 *
Redundancy	4.1	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol