1P2F
Crystal Structure Analysis of Response Regulator DrrB, a Thermotoga maritima OmpR/PhoB Homolog
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.890, 59.390, 77.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.220 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 * | 0.320 * |
| Total number of observations | 424245 * | |
| Number of reflections | 44556 | |
| Completeness [%] | 97.4 | 87.6 |
| Redundancy | 7.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 27 * | sodium and potassium phosphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 46.5 (mg/ml) | |
| 2 | 1 | drop | Tris-Cl | 12.5 (mM) | pH8.0 |
| 3 | 1 | drop | 1.0 (M) | ||
| 4 | 1 | drop | dithioerythritol | 10 (mM) | |
| 5 | 1 | reservoir | sodium potassium phospahte | 1.2-1.4 (M) | pH6.3 |
