1P28
The crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with a component of the pheromonal blend: 3-hydroxy-butan-2-one.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.919214 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.217, 62.210, 45.117 |
| Unit cell angles | 90.00, 92.47, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.14508 |
| Rwork | 0.141 |
| R-free | 0.14100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.046 * | 0.182 * |
| Number of reflections | 23078 | |
| <I/σ(I)> | 10.1 | 3.7 |
| Completeness [%] | 97.0 | 93.1 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 293 | Sulzenbacher, G., (2002) Acta Cryst., D58, 2109. * |
