1P26
Crystal structure of zinc(II)-d(GGCGCC)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-08-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.800, 68.800, 55.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 * |
| R-factor | 0.215 |
| Rwork | 0.210 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB ENTRY BD0040 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 3.000 |
| High resolution limit [Å] | 2.900 * | 2.920 |
| Rmerge | 0.110 * | 0.600 * |
| Total number of observations | 133922 * | |
| Number of reflections | 3115 | |
| <I/σ(I)> | 17.8 | 1.8 |
| Completeness [%] | 97.5 | 87.7 |
| Redundancy | 7.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | DNA, TAPS-HCl, ZnCl2, MPD, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Taps-HCl | ||
| 2 | 1 | 1 | ZnCl2 | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hexamer | 2 (mM) | |
| 2 | 1 | drop | TAPS-HCl | 25 (mM) | pH8.0 |
| 3 | 1 | drop | 2.25 (mM) | ||
| 4 | 1 | drop | 1 (%) | ||
| 5 | 1 | reservoir | TAPS-HCl | 40 (mM) | pH8.0 |
| 6 | 1 | reservoir | 2.25 (mM) | ||
| 7 | 1 | reservoir | MPD | 30 (%) |
