1P1X
Comparison of class I aldolase binding site architecture based on the crystal structure of 2-deoxyribose-5-phosphate aldolase determined at 0.99 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 85 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.783 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.730, 42.010, 145.410 |
| Unit cell angles | 90.00, 98.53, 90.00 |
Refinement procedure
| Resolution | 8.000 - 0.990 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.031 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 0.990 |
| Number of reflections | 314901 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 13-18% MPEG 5000, 0.1M cacodylate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
