1P1O

Crystal structure of the GluR2 ligand-binding core (S1S2J) mutant L650T in complex with quisqualate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	CCD
Collection date	2001-03-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.920
Spacegroup name	P 21 21 2
Unit cell lengths	63.978, 90.865, 47.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.600
R-factor	0.207
Rwork	0.204
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.191 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.650
High resolution limit [Å]	1.600	1.600
Rmerge	0.079	0.097
Number of reflections	36062
<I/σ(I)>	19.1
Completeness [%]	97.1	85.5
Redundancy	3.79

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	4 *	PEG4000, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	14-18 (%)
2	1	reservoir	ammonium sulfate	0.2-0.4 (M)