1P1G

MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH PRO-1 MUTATED TO GLY-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	133
Collection date	1997-12
Spacegroup name	P 31 2 1
Unit cell lengths	95.810, 95.810, 104.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
Rwork	0.227
R-free	0.27000
Structure solution method	OTHER
RMSD bond length	0.009
RMSD bond angle	1.550
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.790
High resolution limit [Å]	2.500	2.500
Rmerge	0.049 *	0.105 *
Total number of observations	50378 *
Number of reflections	18195 *
<I/σ(I)>	6.5
Completeness [%]	93.7	94.8
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	PEG400	2 (%)