1P13
Crystal Structure of the Src SH2 Domain Complexed with Peptide (SDpYANFK)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-11 |
| Detector | MACSCIENCE DIP100S |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.384, 58.793, 62.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.880 - 1.600 * |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.029 |
| RMSD bond angle | 30.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.900 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.630 |
| Rmerge | 0.057 * | 0.423 * |
| Total number of observations | 234548 * | |
| Number of reflections | 27413 | |
| Completeness [%] | 98.0 | 69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 8000, sodium acetate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 30 (%(w/v)) | |
| 2 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | protein | 18 (mg/ml) |
