1P0X
F393Y mutant heme domain of flavocytochrome P450 BM3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-09 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8020 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.608, 152.983, 61.774 |
Unit cell angles | 90.00, 94.54, 90.00 |
Refinement procedure
Resolution | 17.000 - 2.000 |
Rwork | 0.172 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 2HPD |
RMSD bond length | 0.013 |
RMSD bond angle | 2.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.085 | 0.183 |
Total number of observations | 837703 * | |
Number of reflections | 67437 | |
<I/σ(I)> | 10 | 2.15 |
Completeness [%] | 91.0 | 85.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | PEG 8000, PIPES, MAGNESIUM SULFATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium PIPES | 100 (mM) | pH6.5-7.5 |
2 | 1 | reservoir | 40 (mM) | ||
3 | 1 | reservoir | PEG8000 | 18-21 (%) | |
4 | 1 | drop | protein | 40 (mg/ml) | |
5 | 1 | drop | Tris-HCl | 50 (mM) | |
6 | 1 | drop | EDTA | 1 (mM) | pH7.4 |