1P0C

Crystal Structure of the NADP(H)-Dependent Vertebrate Alcohol Dehydrogenase (ADH8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.953
Spacegroup name	C 1 2 1
Unit cell lengths	122.551, 79.523, 91.892
Unit cell angles	90.00, 113.10, 90.00

Refinement procedure

Resolution	30.000 - 2.200
Rwork	0.198
R-free	0.24000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.310 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200	2.100 *
Rmerge	0.113 *	0.447 *
Total number of observations	150436 *
Number of reflections	79197
Completeness [%]	98.4	99 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293 *	Valencia, E., (2003) Acta Crystallogr., Sect.D, 59, 334. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)