1P0B

Crystal Structure Of tRNA-Guanine Transglycosylase (TGT) From Zymomonas mobilis Complexed With Archaeosine Precursor, Preq0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-03-06
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	90.620, 65.540, 70.330
Unit cell angles	90.00, 96.46, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.176
Rwork	0.176
R-free	0.20000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1pud
RMSD bond length	0.008
RMSD bond angle	22.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.104 *	0.562 *
Total number of observations	163734 *
Number of reflections	43081
Completeness [%]	95.6	92.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	25 *	Romier, C., (1996) Proteins: Struct.,Funct., Genet., 24, 516. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	100 (mM)
2	1	reservoir	PEG8000	13 (%)
3	1	reservoir	dithiothreitol	1 (mM)
4	1	reservoir	agarose	0.2 (%)
5	1	drop	HEPES	10 (mM)	pH7.5
6	1	drop		2 (M)
7	1	drop	dithiothreitol	1 (mM)
8	1	drop	protein	12 (mg/ml)