1OZY
Crystal Structure of Phospholipase A2 (MIPLA3) From Micropechis Ikaheka
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 41 |
| Unit cell lengths | 53.522, 53.522, 148.272 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.2276 |
| Rwork | 0.228 |
| R-free | 0.28330 |
| Structure solution method | MR |
| Starting model (for MR) | PDB CODE: 1UNE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.825 |
| Data reduction software | DENZO (1.96) |
| Data scaling software | SCALEPACK (1.96) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 11335 | |
| <I/σ(I)> | -3 | |
| Completeness [%] | 98.7 | 88.2 |
| Redundancy | 6.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | ammonium sulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
