1OZB
Crystal Structure of SecB complexed with SecA C-terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.500, 114.500, 286.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.800 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.26800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SecB coordinate |
RMSD bond length | 0.008 |
RMSD bond angle | 25.200 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.056 * | 0.294 * |
Total number of observations | 323553 * | |
Number of reflections | 47436 | |
<I/σ(I)> | 31.3 | 6 |
Completeness [%] | 99.2 | 98.1 |
Redundancy | 6.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.7 | 277 | PEG 2000, magnesium sulfate, benzamidine hydrochloride, glycine , pH 9.7, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 17.4 (mg/ml) | |
2 | 1 | reservoir | PEG2000 | 24.5 (%(w/v)) | |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | glycine | 100 (mM) | pH9.7 |