1OZ7
Crystal structure of Echicetin from the venom of Indian saw-scaled viper (Echis carinatus) at 2.4 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.966, 95.258, 42.611 |
| Unit cell angles | 90.00, 112.58, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.18819 |
| Rwork | 0.185 |
| R-free | 0.24800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj3 |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 2.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.098 | 0.210 |
| Number of reflections | 9776 | |
| <I/σ(I)> | 9 | 2.5 |
| Completeness [%] | 95.0 | 85 * |
| Redundancy | 5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 * | 298 | 100mM Sodium acetate, 2.0M Ammonium sulphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate trihydrate | 0.1 (M) | pH4.6 |
| 2 | 1 | drop | protein | 15 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 5 | 1 | reservoir | 10 (mM) |
