1OYW

Structure of the RecQ Catalytic Core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	110
Detector technology	CCD
Collection date	2003-01-13
Detector	CUSTOM-MADE
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	66.696, 54.589, 78.830
Unit cell angles	90.00, 110.85, 90.00

Refinement procedure

Resolution	20.000 * - 1.800
R-factor	0.213
Rwork	0.193
R-free	0.24400 *
Structure solution method	SAD
RMSD bond length	0.006 *
RMSD bond angle	1.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.000	1.890
High resolution limit [Å]	1.800	1.800
Rmerge	0.081 *	0.442 *
Total number of observations	353669 *
Number of reflections	51478
<I/σ(I)>	16.3	4.6
Completeness [%]	99.7	99.7
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	PEG 1000, PEG 400, MES, Ammonium Sulfate, Manganese Chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		100 (mM)
3	1	reservoir	PEG1000	0.5 (%)
4	1	reservoir	PEG400	2-4 (%)
5	1	reservoir	MES	50 (mM)	pH6.5
6	1	reservoir	ammonium sulfate	50 (mM)