1OYW
Structure of the RecQ Catalytic Core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-01-13 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.696, 54.589, 78.830 |
| Unit cell angles | 90.00, 110.85, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| R-factor | 0.213 |
| Rwork | 0.193 |
| R-free | 0.24400 * |
| Structure solution method | SAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.081 * | 0.442 * |
| Total number of observations | 353669 * | |
| Number of reflections | 51478 | |
| <I/σ(I)> | 16.3 | 4.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 1000, PEG 400, MES, Ammonium Sulfate, Manganese Chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | reservoir | PEG1000 | 0.5 (%) | |
| 4 | 1 | reservoir | PEG400 | 2-4 (%) | |
| 5 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
| 6 | 1 | reservoir | ammonium sulfate | 50 (mM) |
