1OYU
Long-Distance conformational changes in a protein engineered by modulated sequence duplication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.350, 60.350, 213.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 2.500 |
| R-factor | 0.219 |
| Rwork | 0.209 |
| R-free | 0.32400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lzm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.620 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 * | |
| Number of reflections | 12441 * | |
| <I/σ(I)> | 8.2 | 3.3 |
| Completeness [%] | 94.8 | 96.6 |
| Redundancy | 6.8 | 5.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 | 4 * | 25% poly-ethylene glycol 4000, 50mM phosphate buffer, 0.2mM ammonium acetate, 20% isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
| 2 | 1 | reservoir | isopropanol | 20 (%(v/v)) | |
| 3 | 1 | reservoir | phosphate buffer | 50 (mM) | pH5.5 |
| 4 | 1 | reservoir | ammonium acetate | 0.2 (mM) |
