1OY5

Crystal structure of tRNA (m1G37) methyltransferase from Aquifex aeolicus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9640,0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	153.351, 96.139, 57.426
Unit cell angles	90.00, 96.24, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.28048
Rwork	0.279
R-free	0.30000 *
Structure solution method	MAD
RMSD bond length	0.018 *
RMSD bond angle	1.970 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.580	2.580
Number of reflections	31977
Completeness [%]		96.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	278	Jancarik, J., (1991) J. Appl. Crystallogr., 24, 409. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium cirtate	0.1 (M)	pH5.5
3	1	reservoir	PEG3000	20 (%)