1OXX
Crystal structure of GlcV, the ABC-ATPase of the glucose ABC transporter from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.282, 48.427, 183.533 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.500 * - 1.500* |
R-factor | 0.17449 |
Rwork | 0.172 |
R-free | 0.21200 * |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.500 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.075 * | 0.373 * |
Number of reflections | 62479 | |
<I/σ(I)> | 14.3 | 2 |
Completeness [%] | 80.0 | 31.2 |
Redundancy | 2.93 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 * | Verdon, G., (2002) Acta Crystallogr., D58, 362. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.25 (mg/ml) | |
2 | 1 | reservoir | PEG3350 | 15 (%) | |
3 | 1 | reservoir | PEG400 | 15 (%) | |
4 | 1 | reservoir | Tris | 0.1 (M) | pH8.3 |
5 | 1 | reservoir | 0.5 (M) | ||
6 | 1 | reservoir | glycerol | 5 (%) |