1OXS
Crystal structure of GlcV, the ABC-ATPase of the glucose ABC transporter from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.028, 48.267, 183.037 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 * - 1.650 |
R-factor | 0.16867 |
Rwork | 0.165 |
R-free | 0.20900 * |
Structure solution method | experimental phases derived from SAD data (iodide ions) |
RMSD bond length | 0.016 |
RMSD bond angle | 1.620 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.068 * | 0.373 * |
Number of reflections | 49451 | |
<I/σ(I)> | 22.4 | 5.9 |
Completeness [%] | 98.1 | 90.7 |
Redundancy | 5.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 291 | PEG 3350, PEG 400, Tris, NaI, glycerol, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.25 (mg/ml) | |
10 | 1 | reservoir | glycerol | 15 (%) | |
2 | 1 | drop | MES | 20 (mM) | pH6.5 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | glycerol | 5 (%(v/v)) | |
5 | 1 | drop | 5 (mM) | ||
6 | 1 | reservoir | PEG3350 | 15 (%(w/v)) | |
7 | 1 | reservoir | PEG400 | 15 (%(w/v)) | |
8 | 1 | reservoir | Tris | 0.1 (M) | pH8.3 |
9 | 1 | reservoir | 0.5 (M) |