1OXJ
Crystal structure of the Smaug RNA binding domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2001-03-01 |
Detector | SBC-2 |
Wavelength(s) | 0.94207, 0.97941, 0.97927 |
Spacegroup name | H 3 |
Unit cell lengths | 129.320, 129.320, 33.125 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 1.800 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.24400 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.010 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.036 | 0.330 |
Total number of observations | 151643 * | |
Number of reflections | 19192 | |
<I/σ(I)> | 34.9 | 3.2 |
Completeness [%] | 95.5 | 74.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | PEG 4000, Sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | PEG 4000, Sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 17 (%) | |
2 | 1 | reservoir | sodium acetate | 220 (mM) | |
3 | 1 | reservoir | Tris-Cl | 100 (mM) | pH8.0 |