1OXE
Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.952, 62.768, 69.519 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 1.690* |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.24300 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.586 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 * | 1.220 |
High resolution limit [Å] | 1.690 * | 1.690 * |
Rmerge | 0.043 * | 0.330 * |
Number of reflections | 73514 | |
Completeness [%] | 90.8 * | 83.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | GdFP | 9.1 (mg/ml) | |
3 | 1 | drop | PEG1000 | 14 (%(w/v)) | |
4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
5 | 1 | reservoir | PEG8000 | 5 (%) | |
6 | 1 | reservoir | ethylene gylcol | 10 (%) |