1OXB
Complex between YPD1 and SLN1 response regulator domain in space group P2(1)2(1)2(1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.730, 74.250, 98.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.300 |
R-factor | 0.219 |
Rwork | 0.214 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1QSP (YPD1) |
RMSD bond length | 0.012 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.050 | 0.239 * |
Total number of observations | 84027 * | |
Number of reflections | 17319 * | |
<I/σ(I)> | 19 | |
Completeness [%] | 98.3 | 94.5 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 296 | Choobakc, L., (2003) Acta Cryst., D59, 927. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-12 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2-3 (M) | |
3 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.3 |