1OX9
Crystal structure of SspB-ssrA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9500 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.513, 81.524, 131.773 |
Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.245 |
Rwork | 0.243 |
R-free | 0.29700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.430 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.990 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.124 * | 0.236 * |
Total number of observations | 86232 * | |
Number of reflections | 34398 | |
Completeness [%] | 93.4 | 89.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 22 * | PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.7 |
3 | 1 | drop | 250 (mM) | ||
4 | 1 | drop | EDTA | 2 (mM) | |
5 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
6 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
7 | 1 | reservoir | magnesium acetate | 200 (mM) | |
8 | 1 | reservoir | PEG8000 | 15-18 (%) |