1OX8
Crystal structure of SspB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.116, 60.116, 187.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.249 |
| Rwork | 0.248 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 * | 0.196 * |
| Total number of observations | 503846 * | |
| Number of reflections | 18454 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 * | 22 * | NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.7 |
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 6 | 1 | reservoir | HEPES-KOH | 100 (mM) | pH7.5 |
| 7 | 1 | reservoir | 4.0-4.1 (M) |
