1OX7
Crystal structure of yeast cytosine deaminase apo-enzyme: inorganic zinc bound
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.491, 70.467, 71.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.430 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.17900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.370 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.480 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.042 * | 0.165 |
| Number of reflections | 98785 | |
| <I/σ(I)> | 27.4 | 9.5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 4. * | PEG 8000, calcium acetate, sodium cacodylate, pH 6.5, streak seeding, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG8000 | 30 (%) | |
| 2 | 1 | drop | sodium acetate | 0.2 (M) | |
| 3 | 1 | drop | sodium cacodylate | 0.1 (M) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG8000 | 22-25 (%) | |
| 6 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
