1OX0
The crystal structure of beta-ketoacyl-[acyl carrier protein] synthase II from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979190 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.666, 89.973, 62.202 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.300 |
| R-factor | 0.13825 |
| Rwork | 0.138 |
| R-free | 0.16000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.810 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.080 * | 0.351 * |
| Total number of observations | 1002530 * | |
| Number of reflections | 88233 * | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 8.5 * | 7.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 292 | PEG 3350, magnesium acetate, Tris HCl, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 392K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.5 |
| 2 | 1 | reservoir | EDTA | 1 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | protein | 20 (mg/ml) |
