1OWK
Substituted 2-Naphthamidine Inhibitors of Urokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 160 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.160, 53.000, 82.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.400 * |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (98.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.660 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 4844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 * | 18-24 * | Nienaber, V., (2000) J.Biol.Chem., 275, 7239. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.15 (M) | ||
| 2 | 1 | reservoir | PEG4000 | 20 (%) | pH4.8-6.0 |
| 3 | 1 | drop | protein | 6 (mg/ml) | |
| 4 | 1 | drop | citrate | 10 (mM) | pH4.0 |
| 5 | 1 | drop | epsilon-aminocaproic acid p-carbethoxyphenyl | 3 (mM) | |
| 6 | 1 | drop | 1 (%) |
