1OWF
Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-27 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.660, 58.832, 181.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.780 - 1.950 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.27500 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 26109 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 19 * | PEG5000-MME, glycerol, Tris-HCl, magnesium chloride, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG5000-MME | ||
| 2 | 1 | 1 | glycerol | ||
| 3 | 1 | 1 | Tris-HCl | ||
| 4 | 1 | 1 | magnesium chloride | ||
| 5 | 1 | 1 | sodium chloride | ||
| 6 | 1 | 2 | PEG5000-MME | ||
| 7 | 1 | 2 | magnesium chloride | ||
| 8 | 1 | 2 | sodium chloride |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.7 (mg/ml) | |
| 10 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | glycerol | 8 (%) | |
| 6 | 1 | reservoir | glycerol | 15 (%) | |
| 7 | 1 | reservoir | PEG5000 MME | 25 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.5 |
| 9 | 1 | reservoir | 10 (mM) |
