1OWD

Substituted 2-Naphthamidine inhibitors of urokinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	160
Detector technology	IMAGE PLATE
Collection date	1998-10-19
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	55.160, 53.000, 82.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.320
R-factor	0.269
Rwork	0.269
R-free	0.31300
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	25.000 *
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR
Refinement software	X-PLOR (98.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	22.290
High resolution limit [Å]	2.320
Number of reflections	9926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *	18-24 *	Nienaber, V., (2000) J.Biol.Chem., 275, 7239. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.15 (M)
2	1	reservoir	PEG4000	20 (%)	pH4.8-6.0
3	1	drop	protein	6 (mg/ml)
4	1	drop	citrate	10 (mM)	pH4.0
5	1	drop	epsilon-aminocaproic acid p-carbethoxyphenyl	3 (mM)
6	1	drop		1 (%)