1OW8
Paxillin LD2 motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.976, 220.780, 97.373 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.500 - 2.850 |
| R-factor | 0.23726 |
| Rwork | 0.235 |
| R-free | 0.27500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBentry 1K05 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.060 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.104 * | 0.680 * |
| Total number of observations | 82912 * | |
| Number of reflections | 22456 | |
| <I/σ(I)> | 5.1 | 1.2 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 18 * | sodium chloride, glycerol, Hepes, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 3.9 (M) | ||
| 2 | 1 | reservoir | glycerol | 5 (%) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH6.3 |
