1OW6

Paxillin LD4 motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-12
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	88.308, 223.274, 96.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.600 * - 2.350
R-factor	0.24326
Rwork	0.242
R-free	0.27300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k05
RMSD bond length	0.012
RMSD bond angle	2.210 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.600	2.480
High resolution limit [Å]	2.350	2.350
Rmerge	0.081 *	0.750 *
Total number of observations	116554 *
Number of reflections	39088
<I/σ(I)>	7.2	1
Completeness [%]	97.3	99.1
Redundancy	3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	18 *	sodium chloride, glycerol, Hepes, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		3.9 (M)
2	1	reservoir	glycerol	5 (%)
3	1	reservoir	HEPES	100 (mM)	pH6.3