1OW2
STRUCTURE AND MECHANISM OF ACTION OF ISOPENTENYLPYROPHOSPHATE-DIMETHYLALLYLPYROPHOSPHATE ISOMERASE: COMPLEX OF C67A MUTANT WITH EIPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.948, 71.394, 92.381 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 * - 2.000 |
R-factor | 0.228 * |
Rwork | 0.228 |
R-free | 0.28200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hzt |
RMSD bond length | 0.008 |
RMSD bond angle | 0.021 |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 6.230 | 2.090 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.066 | 0.128 |
Total number of observations | 97958 * | |
Number of reflections | 32619 * | |
<I/σ(I)> | 6.2 | 4.7 |
Completeness [%] | 98.2 | 98.1 * |
Redundancy | 3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 | PEG 2000, MANGANESE CHLORIDE, AMMONIUM SULFATE, pH 5.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG2000 | 16 (%) | |
3 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | Tris/Maleate | pH5.5 |