1OVP
LecB (PA-LII) in complex with fructose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.060, 69.050, 74.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.400 |
R-factor | 0.1833 |
Rwork | 0.182 |
R-free | 0.19700 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.102 |
RMSD bond angle | 1.561 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.061 * | 0.087 |
Total number of observations | 155542 * | |
Number of reflections | 26791 | |
Completeness [%] | 98.8 * | 99.7 |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG8000, ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 20 (%) | |
2 | 1 | reservoir | ammonium sulfate | 150 (mM) | |
3 | 1 | drop | protein | 5 (mg/ml) | |
4 | 1 | drop | Tris | 100 (mM) | pH8.0 |