1OVL
Crystal Structure of Nurr1 LBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2001-11-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 31 |
Unit cell lengths | 80.383, 80.383, 227.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 500.000 - 2.200 |
R-factor | 0.231 |
Rwork | 0.217 |
R-free | 0.25900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.700 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.072 * | 0.269 |
Total number of observations | 287222 * | |
Number of reflections | 82388 | |
<I/σ(I)> | 6.2 | 2.4 |
Completeness [%] | 98.7 | 91.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.9 * | 20 * | PEG3350,KBr, Hepes, pH 6.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-7 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.9 |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | EDTA | 2 (mM) | |
5 | 1 | drop | dithiothreitol | 5 (mM) | |
6 | 1 | reservoir | PEG3350 | 18 (%(w/v)) | |
7 | 1 | reservoir | HEPES | 0.1 (M) | pH6.5 |
8 | 1 | reservoir | 0.2 (M) |