1OVH

T4 Lysozyme Cavity Mutant L99A/M102Q Bound With 2-Chloro-6-Methyl-Aniline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	UCSD MARK II
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	60.700, 60.700, 97.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	13.000 - 1.950
R-factor	0.185
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lgu
RMSD bond length	0.017
RMSD bond angle	2.800
Data reduction software	San (Diego Multiwire)
Data scaling software	SDMS
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000	2.100
High resolution limit [Å]	1.950	1.950
Rmerge	0.080	0.161
Number of reflections	14679
<I/σ(I)>	11.2	2.3
Completeness [%]	93.0	87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1