1OVD
THE K136E MUTANT OF LACTOCOCCUS LACTIS DIHYDROOROTATE DEHYDROGENASE A IN COMPLEX WITH OROTATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.098 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.870, 108.290, 65.940 |
| Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.250 |
| Rwork | 0.178 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Lactococcus lactis DHODA PDB ID 1DOR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.089 | 0.282 |
| Total number of observations | 146256 * | |
| Number of reflections | 34158 * | |
| <I/σ(I)> | 15.3 | 4.93 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 293 | PEG 6K, Sodium acetate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 0.05 (M) | pH7.0 |
| 3 | 1 | reservoir | 25 (mM) | pH6.0 | |
| 4 | 1 | reservoir | glycerol | 10 (%) |
