1OV9
Crystal structure of the N-terminal dimerisation domain of VicH, the H-NS protein from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9184 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 37.085, 49.192, 101.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.258 |
Rwork | 0.233 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ni8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.085 * | 0.570 * |
Total number of observations | 11950 * | |
Number of reflections | 3894 | |
<I/σ(I)> | 7 | 1.2 |
Completeness [%] | 90.8 * | 90.8 |
Redundancy | 3.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | sodium chloride, PEG 3350, potassium iodide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
2 | 1 | drop | 300 (mM) | ||
3 | 1 | drop | protein | 20 (mg/ml) | |
4 | 1 | reservoir | 0.2 (M) | ||
5 | 1 | reservoir | PEG3350 | 20 (%) |