1OV7
T4 Lysozyme Cavity Mutant L99A/M102Q Bound with 2-Allyl-6-Methyl-Phenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | UCSD MARK II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.800, 60.800, 97.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.000 - 2.000 |
| R-factor | 0.189 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lgu |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.700 |
| Data reduction software | San (Diego Multiwire) |
| Data scaling software | SDMS |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.160 |
| Number of reflections | 15008 | |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 89.0 | 67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
