1OUV
Helicobacter cysteine rich protein C (HcpC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.982, 46.198, 76.390 |
Unit cell angles | 90.00, 120.95, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.202 |
R-free | 0.24900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1klx |
RMSD bond length | 0.005 |
RMSD bond angle | 0.971 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 * | 0.347 * |
Number of reflections | 21245 | |
<I/σ(I)> | 14.37 | 2.14 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 30% PEG 4000, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 30% PEG 4000, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | sodium citrate | 0.1 (M) | |
3 | 1 | reservoir | ammonium acetate | 0.2 (M) | pH6.4 |
4 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |