1OU9
Structure of SspB, a AAA+ protease delivery protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2002-06-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97898, 0.97939, 0.96866, 0.98397 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 65.292, 137.839, 93.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.950 - 1.800 |
R-factor | 0.23 |
Rwork | 0.217 |
R-free | 0.25300 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 25.200 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.068 * | 0.553 * |
Total number of observations | 434385 * | |
Number of reflections | 39686 | |
Completeness [%] | 99.9 * | 100 |
Redundancy | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 20 * | calcium acetate, sodium cacodylate, PEG 6000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.7 (mg/ml) | |
2 | 1 | reservoir | calcium acetate | 0.24 (M) | |
3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH5.6 |
4 | 1 | reservoir | PEG8000 | 12 (%(w/v)) |