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1OU9

Structure of SspB, a AAA+ protease delivery protein

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X4A
Synchrotron siteNSLS
BeamlineX4A
Temperature [K]113
Detector technologyCCD
Collection date2002-06-26
DetectorADSC QUANTUM 4
Wavelength(s)0.97898, 0.97939, 0.96866, 0.98397
Spacegroup nameC 2 2 21
Unit cell lengths65.292, 137.839, 93.790
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution16.950 - 1.800
R-factor0.23
Rwork0.217
R-free0.25300
Structure solution methodMAD
RMSD bond length0.006
RMSD bond angle25.200

*

Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.910
High resolution limit [Å]1.8001.800
Rmerge0.068

*

0.553

*

Total number of observations434385

*

Number of reflections39686
Completeness [%]99.9

*

100
Redundancy10.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5.620

*

calcium acetate, sodium cacodylate, PEG 6000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein2.7 (mg/ml)
21reservoircalcium acetate0.24 (M)
31reservoirsodium cacodylate0.1 (M)pH5.6
41reservoirPEG800012 (%(w/v))

243083

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