1OU6
Biosynthetic thiolase from Zoogloea ramigera in complex with acetyl-O-pantetheine-11-pivalate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.380, 78.950, 148.820 |
| Unit cell angles | 90.00, 92.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.070 |
| R-factor | 0.16307 |
| Rwork | 0.161 |
| R-free | 0.21171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.432 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 111939 | |
| <I/σ(I)> | 9.5 | 4.5 |
| Completeness [%] | 93.9 | 83.6 |
| Redundancy | 2.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 288 | ammonium sulfate, lithium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
