1OU5
Crystal structure of human CCA-adding enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 102.520, 102.520, 206.658 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.400 |
| R-factor | 0.282 |
| Rwork | 0.278 |
| R-free | 0.31800 |
| Structure solution method | SIR |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.069 * | 0.273 * |
| Total number of observations | 46729 * | |
| Number of reflections | 17538 * | |
| <I/σ(I)> | 9 | 2.1 |
| Completeness [%] | 99.1 * | 98.9 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 18 * | 100mM sodium citrate, 2.2M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 2 | 1 | drop | 400 (mM) | ||
| 3 | 1 | drop | 0.02 (%(w/v)) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | protein | 6 (mg/ml) | |
| 6 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 7 | 1 | reservoir | ammonium sulfate | 2.2 (M) |
