1OU4
Native PNP +Talo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2001-12-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.948 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 121.615, 121.615, 243.225 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 * - 2.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.350 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.264 |
| Number of reflections | 37511 * | |
| <I/σ(I)> | 7.7 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 295 | 50mM sodium Citrate, 30% ammonium sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | pH8.0 | |
| 2 | 1 | drop | protein | 35 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 30 (%) | |
| 4 | 1 | reservoir | citrate | 50 (mM) | pH5.4 |
