1OTY
Native PNP +ALLO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2001-12-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.948 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 119.970, 119.970, 240.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 * - 2.500 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.24400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.350 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.074 | 0.217 * |
Number of reflections | 36116 | |
<I/σ(I)> | 7.9 | 7.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.7 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 295 | 50mM sodium citrate, 30% ammonium sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 10 (mM) | pH8.0 | |
2 | 1 | drop | protein | 35 (mg/ml) | |
3 | 1 | reservoir | ammonium sulfate | 30 (%) | |
4 | 1 | reservoir | citrate | 50 (mM) | pH5.4 |