1OTJ
Crystal structure of APO (iron-free) TauD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-21 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.848, 117.845, 118.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.221 |
| Rwork | 0.212 |
| R-free | 0.25400 * |
| Structure solution method | Direct methods and MAD |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.340 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 * | 0.334 * |
| Number of reflections | 99459 | |
| <I/σ(I)> | 15.4 | 3.1 |
| Completeness [%] | 96.9 * | 78.9 * |
| Redundancy | 5.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 22 * | PEG 4000, Isopropanol, Sodium citrate, pH 8.0, Batch, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris | 10 (mM) | pH8.0 |
| 2 | 1 | 1 | EDTA | 100 (mM) | pH8.0 |
| 3 | 1 | 1 | nitrogen | 95 (%) | |
| 4 | 1 | 1 | hydrogen gas | 5 (%) |
