1OTG
5-CARBOXYMETHYL-2-HYDROXYMUCONATE ISOMERASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Detector technology | IMAGE PLATE |
Collection date | 1989 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 90.300, 90.300, 129.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.179 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.051 |
Data reduction software | MOSFLM |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.049 |
Number of reflections | 28779 |
Completeness [%] | 89.4 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 100 (mM) | |
2 | 1 | drop | PEG200 | 1 (%) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | ammonium sulfate | 3 (M) | |
5 | 1 | reservoir | ammonium sulfate | 2.5-2.8 (M) | |
6 | 1 | reservoir | Tris-HCl | 10 (mM) |