1OTF
4-OXALOCROTONATE TAUTOMERASE-TRICLINIC CRYSTAL FORM
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.600, 51.500, 51.600 |
| Unit cell angles | 60.00, 81.40, 69.60 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.202 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.047 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.059 |
| Number of reflections | 24515 |
| Completeness [%] | 93.6 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 100 (mM) | |
| 2 | 1 | drop | PEG200 | 1 (%) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 3 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.5-2.8 (M) | |
| 6 | 1 | reservoir | Tris-HCl | 10 (mM) |
