1OTC
THE O. NOVA TELOMERE END BINDING PROTEIN COMPLEXED WITH SINGLE STRAND DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.500, 94.500, 426.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.29200 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 25.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.084 * | 0.498 * |
| Total number of observations | 70514 * | |
| Number of reflections | 24061 | |
| <I/σ(I)> | 10.3 | 1.8 |
| Completeness [%] | 82.5 | 57.1 |
| Redundancy | 2.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | drop contains the same amount of reservoir solution except 50mM NaCl * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NACL | ||
| 2 | 1 | 1 | EDTA | ||
| 3 | 1 | 1 | NAN3 | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 2 | ETHYLENE GLYCOL | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 2 | MES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | alpha subunit | 0.2 (M) | |
| 10 | 1 | reservoir | PEG4000 | 3-8 (%) | |
| 11 | 1 | reservoir | MES | 40 (mM) | |
| 12 | 1 | reservoir | 0.02 (%) | ||
| 13 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 14 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | drop | beta subunit | 1.0-1.5 equivalents each of | |
| 3 | 1 | drop | ssDNA | ||
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | Tris-HCl | 5 (mM) | |
| 6 | 1 | drop | EDTA | 0.1 (mM) | |
| 7 | 1 | drop | 0.02 (%) | ||
| 8 | 1 | drop | dithiothreitol | 2 (mM) | |
| 9 | 1 | reservoir | ethylene glycol | 30 (%) |
