1OTA
E46Q MUTANT OF PHOTOACTIVE YELLOW PROTEIN, P63 AT 295K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2001-01-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 66.917, 66.917, 41.002 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.100 |
R-factor | 0.153 |
Rwork | 0.151 |
R-free | 0.18000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2phy |
RMSD bond length | 0.016 |
RMSD bond angle | 2.440 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.051 | 0.200 |
Number of reflections | 42583 | |
<I/σ(I)> | 20 | 4.8 |
Completeness [%] | 86.0 | 71.7 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | ammonium sulphate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |